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Journal: Journal of Clinical Microbiology
Article Title: Real-Time PCR Differential Detection of Neorickettsia findlayensis and N. risticii in Cases of Potomac Horse Fever
doi: 10.1128/jcm.00250-22
Figure Lengend Snippet: Phylogenetic analysis of Neorickettsia SSA proteins. Neorickettsia SSA proteins were aligned using Clustal Omega, and the phylogenetic tree was constructed with MegAlign Pro. Bootstrap values were calculated by maximum likelihood using RAxML options. The bar indicates sequence distances: the number of amino acid substitutions per site. N. helminthoeca encodes only one SSA protein (333 amino acid residues), whereas Neorickettsia sp. 179522 (endosymbiont of Fasciola hepatica ) encodes two smaller SSA proteins (SSA1a: 197 residues, and SSA1b: 344 residues) that are most closely related to N. findlayensis SSA1. Abbreviations and NCBI accession numbers: Nfin17 ( N. findlayensis Fin17) – SSA1: WP_160095937 , SSA2: WP_160095941 , SSA3, WP_160095943.1 ; NRI ( N. risticii Illinois) – SSA1: WP_015816716 , SSA2: WP_015816703 , SSA3, WP_015816717 ; NSE ( Neorickettsia sennetsu Miyayama) – SSA1: WP_011452276.1 , SSA3: WP_011452279.1 ; NHO ( N. helminthoeca Oregon) – SSA: WP_038560160 ; Nfh ( Neorickettsia sp. 179522) – SSA1a: WP_067980232 , SSA1b: WP_067980235 .
Article Snippet: To estimate confidence levels in phylogenetic analysis, bootstrap values for 1,000 replicates were calculated by Maximum likelihood using RAxML option by
Techniques: Construct, Sequencing